Since then it is recognised that, extraction of the protein from this CPH has immense economic advantages for the cocoa producer countries and it can reduce some of the environmental problems as well. In 90s, some researchers chemical analysis of CPH showed high percentage of crude protein, between 70 and 90 g/kg (Donkoh et al. It may be a significant source of disease inocula, such as black pod rot (Figuiera et al. For each ton of dry beans produced, ten tons of cocoa pod husks are generated, which presents a serious challenge for waste management (Figuiera et al. Approximately 52-76 % of the weight of the cocoa fruit is pod husk (Donkoh et al. Keywords: Cocoa pod husk, Hydrolase enzymes, CLEA-lipase, Face centered central composite design, Response surface methodologyĬocoa pod husk (CPH) is a waste by-product of the cocoa industry, obtained after the removal of the cocoa beans from the fruit. Immobilization effectively improved the stability of lipase against various organic solvents. Referring to the results of OFAT, CLEA-lipase was prepared in the best condition at the presence of 30 mM ammonium sulphate, 70 mM glutaraldehyde with 0.23 mM Bovine serum albumin as an additive. For the production of immobilized biocatalyst, the technique of CLEA is considered as an effective technique for its industrially attractive advantages. The optimum condition of extraction is used for preparation of cross-linked enzyme aggregate (CLEA). The condition that was optimum for the three major factors was achieved using Face centered central composite design (FCCCD) with response surface methodology (RSM) to obtain the highest enzyme activity of crude lipase from CPH. The extraction of high active lipase from CPH has been done under optimum conditions. Among these hydrolases, lipase was chosen for the next steps of experiments as it has a lot of applications in different industries. In this study, seven hydrolase enzymes (amylase, fructosyltransferase, mannanase, glucosidase, glucanase, lipase and protease) were screened from CPH for the first time for feasible industrial production. This study is aimed to utilize this protein source in hydrolase enzyme production. The analysis of CPH has shown that it contains high amounts of protein. Box 10, 50728 Kuala Lumpur, MalaysiaĬocoa pod husk (CPH) is a by-product of cocoa production obtained after removing the beans from the fruit. Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, PO. SpringerPlus (2016) 5:57 DOI 10.1186/S4006-3Ĭocoa pod husk, a new source of hydrolase enzymes for preparation of cross-linked enzyme aggregateįaridah Yusof, Soofia Khanahmadi*, Azura Amid and Safa Senan MahmodĬorrespondence: sophia.
0 Comments
Leave a Reply. |